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Separation of keto acid 2,4-dinitrophenylhydrazones by thin-layer electrophoresis

โœ Scribed by J.R.L. Walker; Elizabeth A. Coop


Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
221 KB
Volume
92
Category
Article
ISSN
1873-3778

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๐Ÿ“œ SIMILAR VOLUMES


Thin-layer chromatography of keto acid 2
โœ Nakao Ariga ๐Ÿ“‚ Article ๐Ÿ“… 1972 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 334 KB

Thin-layer chromatography of keto acid 2,4-dinitrophenylhydrazones was carried out on plate coated with silica gel and sodium bicarbonate. A clear separation was obtained between most of the various keto acid hydrazones. The hydrazone was eluted from the plate with methanol containing 1% concentrate

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For characterizing keto acids, paper chromatography of their DNPHa is most widely used irrespective of the disadvantages (1)) some of which are as follows: According to the methods reported before 1962, the DNPH formed by the usual method were extracted from water solution with an organic solvent su