Determination of phenobarbital and p-hydroxyphenobarbital by densitometry of thin layers

✍ Scribed by S.S. Levin; M.F. Schwartz; D.Y. Cooper; J.C. Touchstone

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 239 KB
• Volume: 154
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)98494-2

✦ Synopsis

Duriug studies of cytochrome P450 activity in rats by induction with phenobarbital (PB), it became of significance to determine methods of detection and quantitation of the drug and its metabohte, p-hydroxyphenobarbital (PPB). Previous attempts at separation have been carried out by gas chromatography which necessitated derivatixation of the compound9. In addition, gas chromatography has proved insensitive for detection of PPB under the conditions investigated.

The present report describes densitometry in situ of thin-layer chromatograms for quantitation of both PB and PPB without derivatization. The method proved sensitive for detection in various rat tissues and excretions, indicating that with proper usage, quantitation of the compounds by thin-layer densitometry can be both reproducib!e and reliable.