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Determination of putrescine in brain tissue using gas chromatography-mass spectrometry

✍ Scribed by T. Noto; T. Hasegawa; H. Kamimura; J. Nakao; H. Hashimoto; T. Nakajima

Publisher: Elsevier Science
Year: 1987
Tongue: English
Weight: 327 KB
Volume: 160
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(87)90063-7

No coin nor oath required. For personal study only.

✦ Synopsis

We used gas chromatography-mass spectrometry to assay putrescine in minute regions of single rat brains. Acid extraction, partial purification on Amberlite CG 120, and derivatization with pentafluoropropionic anhydride preceded the gas chromatography-mass spectrometry. A moving-needle solventless system and a direct inlet system were also used to increase sensitivity. Putrescine was measured accurately at the picomole level; the mean concentration of this polyamine in five regions of rat brain found by this method was 2.7-3.8 times higher than reported by other researchers. o 1987 Academic press, h.

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