Dermal fibroblasts from different layers of human skin are heterogeneous in expression of collagenase and types I and III procollagen mRNA
β Scribed by Mohammad Ali Bahar; Barbara Bauer; Edward E. Tredget; Aziz Ghahary
- Book ID
- 110760007
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 308 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1067-1927
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Understanding the mechanisms responsible fin-photodamage to the skin is most important fl~r dermatology. 3-D cullures have been used as tools to mimic the in vivo situation lot several years. Wc irradiated such a system containing human dermal fibroblasts cultured m collagen gels, a well-known model
In vitro human skin fibroblasts aging was characterized by a continuous increase of collagenase mRNA levels. On the contrary, TIMPβ1 mRNA level decreased only at late passages (> 65% of proliferative life span). Type I and III mRNA levels showed a high variability depending on cell strains studied.