Effects of simulated solar radiation on type I and type III collagens, collagenase (MMP-1) and stromelysin (MMP-3) gene expression in human dermal fibroblasts cultured in collagen gels

✍ Scribed by Laurent Pascual-Le Tallec; Carla Korwin-Zmijowska; Monique Adolphe

• Publisher: Elsevier Science
• Year: 1998
• Tongue: English
• Weight: 700 KB
• Volume: 42
• Category: Article
• ISSN: 1011-1344
• DOI: 10.1016/s1011-1344(98)00075-x

✦ Synopsis

Understanding the mechanisms responsible fin-photodamage to the skin is most important fl~r dermatology. 3-D cullures have been used as tools to mimic the in vivo situation lot several years. Wc irradiated such a system containing human dermal fibroblasts cultured m collagen gels, a well-known model considered Io be a dermal equivalent, which reproduces the interaction between cells and the surrounding extracellular matrix. The effects of solar irradiation ( 315-800 nm ) on file steady-state levels of the mRNAs of extracellular matrix components ( type I and III collagens) and their degrading enzymes (interstitial collagenase, MMP-I and stromelysin 1. MMP-3) were measured. Exposure to tow levels of solar radiation (0-10 J cm : in the UVA, i.e. suberythemal UVA doses) caused a transient decrease in type I procollagen mRNA, an increase in MMP-mRNA, and no change in type II1 procollagen rnRNA stead3,-state levels. These results describe lhe cariy changes in the connective tissue of the skin Iollowing exposure to low level solar stimulation, and nlay help explain the lung term changes m photodamaged skin.