Uncoordinate expressions of type I and III collagens, collagenase and tissue inhibitor of matrix metalloproteinase 1 along in vitro proliferative life span of human skin fibroblasts: Regulation by all-trans retinoic acid.
✍ Scribed by Valérie Bizot-Foulon; Brigitte Bouchard; William Hornebeck; Louis Dubertret; Brigitte Bertaux
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 452 KB
- Volume
- 19
- Category
- Article
- ISSN
- 1065-6995
No coin nor oath required. For personal study only.
✦ Synopsis
In vitro human skin fibroblasts aging was characterized by a continuous increase of collagenase mRNA levels. On the contrary, TIMP‐1 mRNA level decreased only at late passages (> 65% of proliferative life span). Type I and III mRNA levels showed a high variability depending on cell strains studied. However, type I and III collagen expressions varied parallely. All‐trans retinoic acid (RA) decreased collagenase expression and stimulated TIMP‐1 expression. Under RA action, high variability in mRNAs levels encoding type I and III collagens was observed with HSF passages. However, RA tended to correct variations in collagens expressions observed along HSF life span.