Cytogenetic Effect of Griseofulvin in Mouse Spermatocytes

✍ Scribed by Maha A. Fahmy; Nagwa H. A. Hassan

• Book ID: 101286604
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 406 KB
• Volume: 16
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/(sici)1099-1263(199603)16:2<177::aid-jat330>3.0.co;2-t

✦ Synopsis

The genotoxic effects of griseofulvin (GF) in mouse primary spermatocytes at diakinesis metaphase I of meiosis were investigated. Griseofulvin was administered orally as a single dose of 500, 1000, 1500 and 2000 mg kg-' body wt. and a multiple treatment with a daily dose of 1009 mg kg-' body wt. for three and five successive doses. Both single and multiple treatment induced a statistically significant increase in the percentage of chromosomal aberrations which have a dose and time-dependent relationship. The frequency of chromosomal aberrations peaked 6 and 12 h post treatment; with the highest dose of the drug it reached 27.8% k 0.87 and 27.66% f 0.48 6 and 12 h respectively, compared with 5.6% + 0.39 and 5.2% k 0.48 for the control.

The types of aberrations recorded were structural, including X-Y and autosomal univalent, gaps, breaks, fragments, chain IV and numerical in the form of diploid, triploid, tetraploid and aneuploid.

The results of this study suggest that griseofulvin has a genotoxic effect in mouse spermatocytes.