Cytogenetic Effect of Some Insecticides in Mouse Spleen

✍ Scribed by Soheir M. Amer; Maha A. Fahmy; Souria M. Donya

• Book ID: 101286596
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 282 KB
• Volume: 16
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/(sici)1099-1263(199601)16:1<1::aid-jat294>3.0.co;2-a

✦ Synopsis

Several insecticides were tested for their ability to induce chromosomal aberrations in mouse spleen. They were injected i.p. in doses representing approximately 1/8-1/10 of the respective LD~,, values. Doses were: DDT, 5.5 mg kg,' body wt.; malathion, 30 mg kg-I body wt.; Dursban, 4 mg kg-I body wt.; Sevin, 7 mg kg-I body wt.; and Lannate, 1 mg kg-I body wt. 'Mitomycin C' at a dose of 1 mg kg-' body wt. was used as a positive control. Mice were sacrificed 6, 24 and 48 h after treatment. DDT, malathion, dursban and lannate caused maximum chromosomal aberrations 24 h after injection, whereas Sevin induced its maximum effect 6 h after the treatment. All the insecticides induced statistically significant chromosomal aberrations even after excluding the number of metaphases with gaps. The results indicate genotoxicity in mouse spleen cells.