The quorum sensing (QS) systems in Gram-negative bacteria are mostly associated with diffusible N-acyl-l-homoserine lactones (AHLs). AHL-degrading enzymes hydrolyze the AHLs into inactive molecules, thereby blocking the QS systems that are closely linked to virulence factor production and biofilm fo
Crystallization and preliminary crystallographic analysis of tetrahydrodipicolinate-N-succinyltransferase
โ Scribed by Binder, David A.; Blanchard, John S.; Roderick, Steven L.
- Book ID
- 101228426
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 299 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0887-3585
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โฆ Synopsis
Crystals of tetrahydrodipicolinate-N-succinyltransferase have been obtained from solutions containing 2-propanol and polyethylene glycol 4,000. These crystals belong to the monoclinic space group P2,, diffract X-rays to a resolution of 2.2 A, and contain one trimer per asymmetric unit.
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Single crystals of arylesterase (EC 3.1.1.2) from Vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. The present crystals diffract up to 2.2 A resolution and belong to monoclinic space group P2(1). The cell dimensions are a = 55.65(1) A, b = 53
## Abstract The recombinant human p38 MAP kinase has been expressed and purified from both __Escherichia coli__ and SF9 cells, and has been crystallized in two forms by the hanging drop vapor diffusion method using PEG as precipitant. Both crystal forms belong to space group __P__2~1~2~1~2~1~. The
## Abstract A recombinant form of __Rhodobacter sphaeroides__ phosphoribulokinase (PRK), expressed in __Escherichia coli__ and isolated by affinity chromatography, was crystallized by the sitting drop vapor diffusion technique using NH~4~H~2~PO~4~ (pH 5.6) as the precipitating agent. PRK crystalliz