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Crystallization and preliminary crystallographic analysis of the N-terminal actin binding domain of human fimbrin

✍ Scribed by Sharon C. Goldsmith; Navin Pokala; Paul Matsudaira; Steven C. Almo

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 25 KB
Volume: 28
Category: Article
ISSN: 0887-3585
DOI: 10.1002/(sici)1097-0134(199707)28:3<452::aid-prot13>3.0.co;2-g

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✦ Synopsis

We have crystallized the Nterminal actin binding domain (ABD1) of human fimbrin, a representative member of the largest class of actin crosslinking proteins. Diffraction from these crystals is consistent with the orthorhombic space group P2 1 2 1 2 1 (a 5 50.03 Å, b 5 61.24 Å, c 5 102.30 Å). These crystals contain one molecule in the asymmetric unit and diffract to at least 1.9 Å resolution. The crystal structure of ABD1 will be the first structure of an actin crosslinking domain. Pro-

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