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Crystallization and preliminary crystallographic analysis of recombinant human p38 MAP kinase

✍ Scribed by S. Pav; D.M. White; S. Rogers; K.M. Crane; C.L. Cywin; J. Hopkins; M.L. Brown; C.A. Pargellis; L. Tong; W. Davidson

Book ID: 105356443
Publisher: Cold Spring Harbor Laboratory Press
Year: 2008
Tongue: English
Weight: 1016 KB
Volume: 6
Category: Article
ISSN: 0961-8368
DOI: 10.1002/pro.5560060126

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✦ Synopsis

Abstract

The recombinant human p38 MAP kinase has been expressed and purified from both Escherichia coli and SF9 cells, and has been crystallized in two forms by the hanging drop vapor diffusion method using PEG as precipitant. Both crystal forms belong to space group __P__2~1~2~1~2~1~. The cell parameters for crystal form 1 are a = 65.2 Å, b = 74.6 Å and c = 78.1 Å. Those for crystal form 2 are a = 58.3 Å, b = 68.3 Å and c = 87.9 Å. Diffraction data to 2.0 Å resolution have been collected on both forms.

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