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Crystallization and preliminary X-Ray analysis of recombinant staphylokinase

✍ Scribed by Anja Rabijns; Katrien Baeyens; Hendrik L. De Bondt; Camiel De Ranter

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 23 KB
Volume: 27
Category: Article
ISSN: 0887-3585
DOI: 10.1002/(sici)1097-0134(199701)27:1<160::aid-prot17>3.0.co;2-l

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✦ Synopsis

Diffraction quality crystals of recombinant staphylokinase (STAR) have been grown by the hanging drop vapor diffusion technique from a solution containing MgCl 2 , Tris buffer (pH 8.5), and polyethylene glycol 4000. The crystals belong to the monoclinic space group C2 with unit cell dimensions a ‫؍‬ 60.6 Å, b ‫؍‬ 43.7 Å, c ‫؍‬ 54.3 Å, and ␤ ‫؍‬ 115.6°. A complete native data set to 1.8 Å resolution has been collected using synchrotron radiation.

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