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Crystallization and preliminary X-ray studies of ornithine decarboxylase fromTrypanosoma brucei

✍ Scribed by Grishin, Nick V.; Osterman, Andrei L.; Goldsmith, Elizabeth J.; Phillips, Margaret A.

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 222 KB
Volume: 24
Category: Article
ISSN: 0887-3585
DOI: 10.1002/(sici)1097-0134(199602)24:2<272::aid-prot17>3.0.co;2-k

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✦ Synopsis

Trypanosoma brucei ornithine decarboxylase, expressed and purified from E. coli, has been crystallized b y the vapor diffusion method using P E G 3350 as a precipitant. The crystals belong to the monoclinic space group P2, and have cell constants of a = 66.3 A, b = 151.8 A, c = 83.7 A, and p = 101.2'.

While larger crystals are twinned, smaller crystals (0.4 x 0.3 x 0.05 mm3) are single.

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