✦ LIBER ✦

Crystallization and preliminary X-ray diffraction studies of formylmethanofuran: Tetrahydromethanopterin formyltransferase fromMethanopyrus kandleri

✍ Scribed by Shima, Seigo; Thauer, Rudolf K.; Michel, Hartmut; Ermler, Ulrich

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 315 KB
Volume: 26
Category: Article
ISSN: 0887-3585
DOI: 10.1002/(sici)1097-0134(199609)26:1<118::aid-prot12>3.0.co;2-j

No coin nor oath required. For personal study only.

✦ Synopsis

Formy1methanofuran:tetrahydromethanopterin formyltransferase from the hyperthermophilic methanogenic Archaeon Methampyrus kandleri (growth temperature optimum 98°C) was crystallized by vapor diffusion methods. Crystal form M obtained with 2-methyl-2,4-pentanediol as precipitant displayed the space group P2, with unit cell parameters o f a = 87.0A, b = 75.4hi,c = 104.7A, and f3 = 113.9" a n d diffracted better than 2 hi resolution. Crystal form P grown from polyethylene glycol 8000 belonged to the space group 14'22 and had unit cell parameters of 157.5 A and 242.1 hi. Diffraction data to 1.73 A were recorded. Crystal form S which was crystallized from (NH,),SO, in the space group 14'22 with unit cell parameters of 151.3 A and 249.5 A diffracted at least to 2.2 hi resolution. All crystal forms probably have four molecules per asymmetric unit and are suitable for X-ray structure analysis. o 1996 Wiley-Liss, hc.

📜 SIMILAR VOLUMES

Crystallization and preliminary x-ray di

Crystallization and preliminary x-ray diffraction studies of human procathepsin L

✍ René Coulombe; Yunge Li; Sachiko Takebe; Robert Ménard; Patrizia Mason; John S. 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 280 KB 👁 2 views

Human procathepsin L has been expressed in the yeast Pichiapastoris and its inactive (Cys25Ser) and unglycosylated (ThrllOAla) mutant purified, concentrated to 4 mglml, and crystallized by vapor diffusion against solution containing 1.4 M (Na,K)PO, buffer, pH 7.8. Crystal size was increased by multi

Purification, crystallization, and preli

Purification, crystallization, and preliminary X-ray diffraction studies of tRNA-guanine transglycosylase fromZymomonas mobilis

✍ Romier, Christophe; Ficner, Ralf; Reuter, Klaus; Suck, Dietrich 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 399 KB 👁 2 views

The tRNA modifying enzyme tRNA-guanine transglycosylase (Tgt) catalyzes the exchange of guanine in the first position of the anticodon with the queuine precursor 7-aminomethyl-7-deazaguanine. Tgt from Zymomo-na8 mobilis has been purified by crystallization and further recrystallized to obtain single

Crystallization and preliminary X-ray di

Crystallization and preliminary X-ray diffraction study of 1,3,8-trihydroxynaphthalene reductase fromMagnaporthe grisea

✍ Andersson, Arnold; Jordan, Doug; Schneider, Gunter; Valent, Barbara; Lindqvist, 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 243 KB 👁 2 views

1,3,8-Trihydroxynaphthalene reductase was crystallized in the presence of NADPH and the inhibitor tricyclazole. The crystals are trigonal, space group P3,21 or its enantiomorph P3,21. Two crystal forms with slightly different cell dimensions were obtained. Form A has unit cell dimensions a = b = 142

Crystallization and preliminary X-ray st

Crystallization and preliminary X-ray studies of ornithine decarboxylase fromTrypanosoma brucei

✍ Grishin, Nick V.; Osterman, Andrei L.; Goldsmith, Elizabeth J.; Phillips, Margar 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 222 KB 👁 2 views

Trypanosoma brucei ornithine decarboxylase, expressed and purified from E. coli, has been crystallized b y the vapor diffusion method using P E G 3350 as a precipitant. The crystals belong to the monoclinic space group P2, and have cell constants of a = 66.3 A, b = 151.8 A, c = 83.7 A, and p = 101.2

Crystallization and preliminary x‐ray cr

Crystallization and preliminary x‐ray crystallographic studies of recombinant bovine neurocalcin δ

✍ Kumar, Vinod D.; Hidaka, Hiroyoshi; Okazaki, Katsuo; Vijay‐Kumar, Senadhi 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 459 KB 👁 1 views

Neurocalcins are novel brainspecific proteins that belong to a new subclass of the EF-hand super-family of calcium binding proteins, defined by the photoreceptor cell-specific protein recoverin (Terasawa et al., J. Biol. Chem. 267:1959619599, 1992). Here we report the purification and crystallizatio

Crystallization and preliminary X-ray cr

Crystallization and preliminary X-ray crystallographic studies of ketosteroid isomerase fromPseudomonas putida biotype B

✍ Oh, Byung-Ha; Kim, Suhng Wook; Ryu, Seong-Eon; Kim, Sang-Soo; Yoon, Mi-Kyung; Ch 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 235 KB 👁 2 views

The A5-3-ketosteroid isomerase from Pseudomonas putida biotype B has been crystallized. The crystals belong to the space group P2,2,2, with unit cell dimensions of a = 36.48 A, b = 74.30 A, c = 96.02 A, and contain one homodimer per asymmetric unit. Native diffraction data to 2.19 A resolution have