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Crystallization and preliminary X-ray crystallographic studies of ketosteroid isomerase fromPseudomonas putida biotype B

✍ Scribed by Oh, Byung-Ha; Kim, Suhng Wook; Ryu, Seong-Eon; Kim, Sang-Soo; Yoon, Mi-Kyung; Choi, Kwan Yong

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 235 KB
Volume: 24
Category: Article
ISSN: 0887-3585
DOI: 10.1002/(sici)1097-0134(199604)24:4<514::aid-prot10>3.0.co;2-o

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✦ Synopsis

The A5-3-ketosteroid isomerase from Pseudomonas putida biotype B has been crystallized. The crystals belong to the space group P2,2,2, with unit cell dimensions of a = 36.48 A, b = 74.30 A, c = 96.02 A, and contain one homodimer per asymmetric unit. Native diffraction data to 2.19 A resolution have been obtained from one crystal at room temperature indicating that the crystals are quite suitable for structure determination by multiple isomorphous replacement. o ISXI Wiley-Liss, Inc.

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