Crystallization and preliminary X-ray crystallographic studies ofL-2-haloacid dehalogenase fromPseudomonas sp. YL

✍ Scribed by Hisano, Tamao; Hata, Yasuo; Fujii, Tomomi; Liu, Ji-Quan; Kurihara, Tatsuo; Esaki, Nobuyoshi; Soda, Kenji

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 336 KB
• Volume: 24
• Category: Article
• ISSN: 0887-3585
• DOI: 10.1002/(sici)1097-0134(199604)24:4<520::aid-prot12>3.0.co;2-n

✦ Synopsis

The dimeric L-2-haloacid dehalogenase from Pseudomonas sp. YL, (subunit mass, 26179 Da), has been crystallized by vapor diffusion, supplemented by repetitive seeding, against a 50 mM potassium dihydrogenphosphate solution (pH 4.5) containing 15% (w/v) polyethylene glycol 8,000 and 1% (v/v) n-propanol. The crystals belong to the monoclinic space group C2 with unit cell dimensions of a = 92.21 A, b = 62.78 A, c = 50.84 A, and p = 122.4", and contain two dehalogenase dimers in the unit cell. They are of good quality and diffract up to 1.5 A resolution.