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Crystallization and preliminary X-ray diffraction study of 1,3,8-trihydroxynaphthalene reductase fromMagnaporthe grisea

✍ Scribed by Andersson, Arnold; Jordan, Doug; Schneider, Gunter; Valent, Barbara; Lindqvist, Ylva

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 243 KB
Volume: 24
Category: Article
ISSN: 0887-3585
DOI: 10.1002/(sici)1097-0134(199604)24:4<525::aid-prot14>3.0.co;2-n

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✦ Synopsis

1,3,8-Trihydroxynaphthalene reductase was crystallized in the presence of NADPH and the inhibitor tricyclazole. The crystals are trigonal, space group P3,21 or its enantiomorph P3,21. Two crystal forms with slightly different cell dimensions were obtained. Form A has unit cell dimensions a = b = 142.6 hi, c = 70.1 hi and form B cell dimensions a = b = 142.6 A, c = 72.9 A. The diffraction pattern of the latter crystal form extends to 2.5 A resolution.

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