Expression, purification, crystallization, and preliminary X-Ray diffraction analysis of the homodimeric bacterial hemoglobin from Vitreoscilla stercoraria
✍ Scribed by Cataldo Tarricone; Sabina Calogero; Alessandro Galizzi; Alessandro Coda; Paolo Ascenzi; Martino Bolognesi
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 34 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0887-3585
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✦ Synopsis
The recombinant homodimeric hemoglobin from the strictly aerobe gram-negative bacterium Vitreoscilla stercoraria has been expressed in Escherichia coli, purified to homogeneity, and crystallized by vapor diffusion techniques, using ammonium sulfate as precipitant. The crystals belong to the monoclinic space group P2 1 and diffract to HIGH resolution. The unit cell parameters are a ؍ 62.9, b ؍ 42.5, c ϭ 63.2 Å,  ؍ 106.6°; the asymmetric unit contains the homodimeric hemoglobin, with a volume solvent content of 42%. Proteins 27:154-156 1997 Wiley-Liss, Inc.