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Crystallization and preliminary x-ray analysis of Escherichia coli peptidyl-tRNA hydrolase

✍ Scribed by Emmanuelle Schmitt; Michel Fromant; Pierre Plateau; Yves Mechulam; Sylvain Blanquet

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 30 KB
Volume: 28
Category: Article
ISSN: 0887-3585
DOI: 10.1002/(sici)1097-0134(199705)28:1<135::aid-prot14>3.0.co;2-k

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✦ Synopsis

Peptidyl-tRNA hydrolase from

Escherichia coli, a monomer of 21 kDa, was overexpressed from its cloned gene pth and crystallized by using polyethylene glycol as precipitant. The crystals are orthorhombic and have unit cell parameters a 5 47.24 Å, b 5 63.59 Å, and c 5 62.57 Å. They belong to space group P2 1 2 1 2 1 and diffract to better than 1.2 Å resolution. The structure is being solved by multiple isomorphous replacement.

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