Crystallization and preliminary x-ray analysis of the flavoenzyme vanillyl-alcohol oxidase from Penicillium Simplicissimum

✍ Scribed by Andrea Mattevi; Marco W. Fraaije; Alessandro Coda; Willem J.H. van Berkel

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 37 KB
• Volume: 27
• Category: Article
• ISSN: 0887-3585
• DOI: 10.1002/(sici)1097-0134(199704)27:4<601::aid-prot12>3.0.co;2-o

✦ Synopsis

Vanillyl-alcohol oxidase catalyses the oxidation of several 4-hydroxybenzyl alcohols by using 8-alpha-(N3-histidyl)-FAD as a covalently bound prosthetic group. Crystals of vanillyl-alcohol oxidase from Penicillium simplicissimum have been grown by using the vapor diffusion technique. The space group was found to be I, with cell dimensions a = b = 140.5 A, c = 132.9 A. Diffraction data have been recorded to 3.2 A resolution by using a laboratory source and to 2.5 A resolution on flash freezing the crystal at the ELETTRA Synchrotron X-ray diffraction beam line.