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Crystallization and preliminary X-ray crystallographic analysis of arylesterase from vibrio mimicus

โœ Scribed by Faik N. Musayev; Ya-Lin Lee; Je1-Fu Shaw; Yen-Chywan Liaw

Publisher
Cold Spring Harbor Laboratory Press
Year
1995
Tongue
English
Weight
271 KB
Volume
4
Category
Article
ISSN
0961-8368

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โœฆ Synopsis

Single crystals of arylesterase (EC 3.1.1.2) from Vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. The present crystals diffract up to 2.2 A resolution and belong to monoclinic space group P2(1). The cell dimensions are a = 55.65(1) A, b = 53.46(1) A, c = 65.79(1) A, and beta = 106.54(1) degrees. There are two molecules of molecular weight 22 kDa in an asymmetric unit with a solvent content of 43%.

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