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Crystallization and preliminary X-ray crystallographic analysis of phosphoribulokinase from Rhodobacter sphaeroides

✍ Scribed by David L. Roberts; Jennifer A. Runquist; Henry M. Miziorko; Jung-Ja P. Kim

Publisher: Cold Spring Harbor Laboratory Press
Year: 1995
Tongue: English
Weight: 914 KB
Volume: 4
Category: Article
ISSN: 0961-8368
DOI: 10.1002/pro.5560041126

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✦ Synopsis

Abstract

A recombinant form of Rhodobacter sphaeroides phosphoribulokinase (PRK), expressed in Escherichia coli and isolated by affinity chromatography, was crystallized by the sitting drop vapor diffusion technique using NH~4~H~2~PO~4~ (pH 5.6) as the precipitating agent. PRK crystallizes in the cubic space group P432, with unit cell parameters a = b = c = 129.55 Å. Based on the assumption of one 32‐kDa monomer per asymmetric unit, the V~m~ value is 2.83 Å^3^/Da. The octameric molecular symmetry is consistent with two planar tetramers stacked in a nearly eclipsed arrangement. A native data set has been collected to 2.6 Å resolution.

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