Insulin can replace the factor(s) in calf serum whose amount is limiting for multiplication in cell culture of chicken embryo fibroblasts and of chicken embryo fibroblasts infected and converted by avian sarcoma virus. In serum-free, insulincontaining medium, converted cells multiply more than do un
Control of cell multiplication in uninfected chicken cells and chicken cells converted by avian sarcoma viruses
✍ Scribed by Howard M. Temin
- Publisher
- John Wiley and Sons
- Year
- 1969
- Tongue
- English
- Weight
- 586 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
The increased multiplication of converted chicken cells in the presence of limited amounts of serum results from more efficient utilization of multiplicationstimulating activity rather than increased binding or uptake of this activity.
X lo5 cells in 60-mm plastic petri dishes (Falcon Plastics) were exposed to 0.2 ml of virus-containing or control medium, and incubated for 40 min. at 38°C in a humidified CO, incubator. Fresh medium was -~
📜 SIMILAR VOLUMES
Glycolysis and multiplication were studied in parallel cultures of uninfected and of avian sarcoma virus-converted chicken and duck cells. It was found that:
## Abstract Clonal lines of murine and avion sarcoma virus‐transformed mammalian cells, in which the viruses do not productively replicate, were studied for the occurrence of spontaneous or chemical‐induced morphologic reversion. From over 10,000 single‐cellderived clones of MSV‐transformed nonprod
## Abstract The major group‐specific antigen (gs‐a) of avian type‐C viruses was detected by radioimmunoassay in all tissue samples and cultured cells from leukosts‐free chickens. Visceral tissues of individual embryos contained 7–36 ng antigen per mg protein. The mean values in adult tissues were 2
## Abstract Chicken fetal antigen (CFA), a membrane antigen present on fetal chicken red blood cells is lost with chicken development, and reappears on the red blood cells of leukemic chickens. Seven avian species were found to possess CFA. A species hierarchy comparing the quantitative expression
## Abstract Hamster cells (BHK21/13) transformed at the permissive temperature by a temperature‐sensitive mutant (FU 19) of the Schmidt‐Ruppin strain of Rous sarcoma virus exhibited a temperature‐dependent expression of virus‐induced surface antigen (VISA) as assayed by mixed haemadsorption test. T