𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Genetic stability of the sarcoma viruses in murine and avian sarcoma virus-transformed nonproducer cells

✍ Scribed by John R. Stephenson; Edward M. Scolnick; Stuart A. Aaronson

Publisher
John Wiley and Sons
Year
1972
Tongue
French
Weight
463 KB
Volume
9
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Clonal lines of murine and avion sarcoma virus‐transformed mammalian cells, in which the viruses do not productively replicate, were studied for the occurrence of spontaneous or chemical‐induced morphologic reversion. From over 10,000 single‐cellderived clones of MSV‐transformed nonproducer cells examined, only one morphologic revertant was detected. This cell line contained a normal murine sarcoma viral genome, suggesting that it arose as a result of mutation of a cellular gene involved in expression of the transformed state. The frequency of morphologic reversion of avian sarcoma virus‐transformed mammalian cell lines was also investigated. The studies indicate that both murine and avian sarcoma viruses present in transformed nonproducer cells can exist in a genetically stable form.

📜 SIMILAR VOLUMES

Expression of viral protein P27 in avian
Expression of viral protein P27 in avian sarcoma virus-transformed mammalian cells and helper-dependent rescue of rous sarcoma virus
✍ M. Popovič; J. Svoboda; J. Suni; A. Vaheri; J. Pontén 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 781 KB

## Abstract Avian sarcoma virus (ASV)‐transformed mammalian cells, known to be either virus‐producing, virogenic or non‐virogenic, were tested for viral gene products: for p27 by radioimmunoassay and for group‐specific (gs) antigens by the complement fixation test. Clones of B77 virus‐producing RBI

Analysis of v-mos encoded proteins in ce
Analysis of v-mos encoded proteins in cells transformed by several related murine sarcoma viruses
✍ Jackie Papkoff; Dr. Mei H.-T. Lai; Tony Hunter 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 942 KB

## Abstract We have used antisera against synthetic peptides to identify and characterize a 37,000 dalton v‐__mos__ encoded protein (p37__mos__) in cells transformed by M‐MuSV 124. p37__mos__, a phosphoprotein, comprises only about 0.0005% of total cellular protein in cell lines transformed by M‐Mu

Cell surface antigen induced by avian tu
Cell surface antigen induced by avian tumor viruses in hamster cells transformed by a temperature-sensitive mutant of rous sarcoma virus
✍ Michèle C. Aupoix; Jean-Michel Biquard; Annie Cachard 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 414 KB 👁 1 views

## Abstract Hamster cells (BHK21/13) transformed at the permissive temperature by a temperature‐sensitive mutant (FU 19) of the Schmidt‐Ruppin strain of Rous sarcoma virus exhibited a temperature‐dependent expression of virus‐induced surface antigen (VISA) as assayed by mixed haemadsorption test. T

Effect of mouse interferon on cell trans
Effect of mouse interferon on cell transformation and virus production in rat cells exogenously infected with moloney murine sarcoma and leukemia viruses
✍ Mahmoud Huleihel; Mordechai Aboud 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 French ⚖ 614 KB

## Abstract Foci of transformed cells, produced by MSV(124), appeared to result only from the primary infection, since this virus stock yielded a virus‐nonproducing infection. On the other hand, the majority of foci scored in MSV/MLV‐infected cultures, were generated by multiple secondary infection

Influence of 5-bromodeoxyuridine on the
Influence of 5-bromodeoxyuridine on the expression of avian GS antigen in mammalian rous sarcoma virus-transformed cells
✍ V. Sovová; H. Sainerová; M. Urbánková; I. Hloěánek 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 384 KB

## Abstract The avian group‐specific (gs) antigen in the virogenic RSV‐transfarmed LW13‐RsKl and LW13‐RsK4 cells is detectable in the perinuclear zone of the cells only by immunofluorescence tests. After treatment of cells with 5‐bromodeoxyuridine (BUdR) it is detectable even by complement‐fixation

Detection of human c-type “helper” virus
Detection of human c-type “helper” viruses in human leukemic bone marrow with murine sarcoma virus-transformed human and rat non-producer cells
✍ Kees Nooter; Peter Bentvelzen; Chris Zurcher; Johng Rhim 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 694 KB

## Abstract Bone‐marrow cells from two leukemic children were co‐cultivated with the canine thymus cell line A 7573. In early passages, C‐type oncornaviruses were released as detected by extracellular reverse transcriptase assay. Co‐cultivation of the infected canine cells with the non‐producing ce