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Cloning and expression of theBacillus circulansendo-β-1,3-1,4-glucanase gene (bglBC1) inEscherichia coli

✍ Scribed by Ji-Yeon Kim; Han-Bok Kim; Dong-Seok Lee


Book ID
110324415
Publisher
Springer Netherlands
Year
2002
Tongue
English
Weight
175 KB
Volume
24
Category
Article
ISSN
0141-5492

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A new gene encoding the 15' -1,3-glucanase&rninarinase) of Bacillus circulans KCTC3004 was cloned into Escherichia colz using pUC19 as a vector. The gene localized in the 5.3 kb PstI DNA fragment was expressed independently of its orientation in the cloning vector showing enzyme activity about 33 ti