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Cloning and expression of a β -1,3-glucanase gene fromBacillus circulansKCTC3004 inEscherichia coli

✍ Scribed by Dong Seok Lee; Hun-Gil Chang

Publisher
Springer Netherlands
Year
1995
Tongue
English
Weight
459 KB
Volume
17
Category
Article
ISSN
0141-5492

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✦ Synopsis

A new gene encoding the 15' -1,3-glucanase&rninarinase) of Bacillus circulans KCTC3004 was cloned into Escherichia colz using pUC19 as a vector. The gene localized in the 5.3 kb PstI DNA fragment was expressed independently of its orientation in the cloning vector showing enzyme activity about 33 times greater than that. produced by the original B. circulans. The optimum pH and temperature of the cloned enzyme were pH 5.4 and 5O"C, respectively. The molecular weight of the enzyme was about 38,000 and the processing of the enzyme molecule within the E. coli cell was not observed. The enzyme hydrolyzed laminarin to produce iaminaritriose, laminaribiose, and glucose as main products, but it was inactive for lichenan, CMC, or xylan.

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