𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Expression inEscherichia coliof a cloned β-glucanase gene fromBacillus amyloliquefaciens

✍ Scribed by Rainer Borriss; Helmut Bäumlein; Jürgen Hofemeister

Publisher
Springer
Year
1985
Tongue
English
Weight
888 KB
Volume
22
Category
Article
ISSN
1432-0614

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Cloning and expression of a β -1,3-gluca
Cloning and expression of a β -1,3-glucanase gene fromBacillus circulansKCTC3004 inEscherichia coli
✍ Dong Seok Lee; Hun-Gil Chang 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 459 KB

A new gene encoding the 15' -1,3-glucanase&rninarinase) of Bacillus circulans KCTC3004 was cloned into Escherichia colz using pUC19 as a vector. The gene localized in the 5.3 kb PstI DNA fragment was expressed independently of its orientation in the cloning vector showing enzyme activity about 33 ti

Expression of a cloned β-glucanase gene
Expression of a cloned β-glucanase gene from Bacillus amyloliquefaciens in an Escherichia coli relA strain after plasmid amplification
✍ Hecker, Michael ;Riethdorf, Sabine ;Bauer, Christiane ;Schroeter, Andreas ;Borri 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 271 KB

Amino acid starvation of cells of the Escherichia coli relA strain, CP79, which cannot accumulate guanosine tetraphosphate (ppGpp) in response to amino acid limitation, increased the pEG1 plasmid content about 5- to 7-fold in comparison with exponentially growing cells (pEG1:pBR322 with an insertion

Cloning and expression of a thermostable
Cloning and expression of a thermostable exo-α-1,4-glucosidase gene fromBacillus stearothermophilusATCC12016 inEscherichia coli
✍ Yukio Takii; Katsuya Daimon; Yuzuru Suzuki 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 856 KB

The gene coding for a thermostable exo-alpha-1,4-glucosidase (alpha-glucoside glucohydrolase: EC 3.2.1.20) of Bacillus stearothermophilus ATCC 12016 was cloned within a 2.8-kb AvaI fragment of DNA using the plasmid pUC19 as a vector and Escherichia coli JM109 as a host. E. coli with the hybrid plasm