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Cloning and expression of anOerskovia xanthineolyticaβ-1,3-glucanase in Escherichia coli

✍ Scribed by O. Salazar; J. Molitor; J.A. Asenjo


Book ID
110225509
Publisher
Springer Netherlands
Year
1999
Tongue
English
Weight
65 KB
Volume
21
Category
Article
ISSN
0141-5492

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A new gene encoding the 15' -1,3-glucanase&rninarinase) of Bacillus circulans KCTC3004 was cloned into Escherichia colz using pUC19 as a vector. The gene localized in the 5.3 kb PstI DNA fragment was expressed independently of its orientation in the cloning vector showing enzyme activity about 33 ti