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Cloning of β-1,3-glucanases expressed during Cichorium somatic embryogenesis

✍ Scribed by Stéphane Helleboid; Audrey Chapman; Theo Hendriks; Dirk Inzé; Jacques Vasseur; Jean-Louis Hilbert

Book ID
110234459
Publisher
Springer
Year
2000
Tongue
English
Weight
165 KB
Volume
42
Category
Article
ISSN
0167-4412

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✍ Dong Seok Lee; Hun-Gil Chang 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 459 KB

A new gene encoding the 15' -1,3-glucanase&rninarinase) of Bacillus circulans KCTC3004 was cloned into Escherichia colz using pUC19 as a vector. The gene localized in the 5.3 kb PstI DNA fragment was expressed independently of its orientation in the cloning vector showing enzyme activity about 33 ti