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Cloning of β-1,3-1,4-glucanase gene fromBacillus licheniformisEGW039 (CGMCC 0635) and its expression inEscherichia coliBL21 (DE3)

✍ Scribed by Da Teng; Jian-hua Wang; Ying Fan; Ya-lin Yang; Zi-gang Tian; Jin Luo; Guan-pin Yang; Fan Zhang

Publisher: Springer
Year: 2006
Tongue: English
Weight: 225 KB
Volume: 72
Category: Article
ISSN: 1432-0614
DOI: 10.1007/s00253-006-0329-2

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A new gene encoding the 15' -1,3-glucanase&rninarinase) of Bacillus circulans KCTC3004 was cloned into Escherichia colz using pUC19 as a vector. The gene localized in the 5.3 kb PstI DNA fragment was expressed independently of its orientation in the cloning vector showing enzyme activity about 33 ti