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Subcloning and functional analysis of aBacillus subtilisβ-1,3-1,4-glucanase gene (bglS) inEscherichia coli

✍ Scribed by Chen Yongqing; Song Daxin; Huang Xingqi; Jiang Hong; Ao Wanyuan; Zheng Weijun


Publisher
Springer
Year
1990
Tongue
English
Weight
387 KB
Volume
21
Category
Article
ISSN
0343-8651

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A new gene encoding the 15' -1,3-glucanase&rninarinase) of Bacillus circulans KCTC3004 was cloned into Escherichia colz using pUC19 as a vector. The gene localized in the 5.3 kb PstI DNA fragment was expressed independently of its orientation in the cloning vector showing enzyme activity about 33 ti