Chromosome studies in stimulated lymphocytes of B-Cell chronic lymphocytic leukemias

✍ Scribed by Naoki Sadamori; Tin Han; Jun Minowada; Elias Cohen; Avery A. Sandberg

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 702 KB
• Volume: 1
• Category: Article
• ISSN: 0278-0232
• DOI: 10.1002/hon.2900010306

✦ Synopsis

Using a sister chromatid differentiation technique, cell cycle study of stimulated lymphocytes of B-Cell chronic lymphocytic leukemia (B-CLL) revealed their cell cycle progression to be similar to that of normal lymphocytes stimulated by T-cell and various polyclonal B-cell activators (PBA). The chromosome constitutions of stimulated lymphocytes in 62 patients with B-CLL were examined using PBA such as Epstein-Barr virus (EBV) and lipopolysaccharide W from E. coli 055:B5 (LPS). Of the 20 patients with abnormal clones, 11 patients had trisomy 12; other less common abnormalities were trisomy 1, 6q -, i(7q), 14q + , trisomy 16, trisomy 18, reciprocal translocations, and marker chromosomes of unknown origin. These findings indicate that trisomy 12 may be a unique and common karyotypic change in B-CLL. The fact that 3 out of 4 patients with marker chromosomes showed stage IV disease may indicate that a clone with a marker is a predictor of an unfavourable prognosis. The near correlation between trisomy 12 and K chains existed (0.05<p<0.10). Trisomy 12 was seen in all 5 patients with monoclonal paraprotein.