Monoclonal antibody studies in B-cell chronic lymphocytic leukemia and allied disorders

✍ Scribed by Alan C. Aisenberg; Barbara M. Wilkes

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 458 KB
• Volume: 1
• Category: Article
• ISSN: 0278-0232
• DOI: 10.1002/hon.2900010104

✦ Synopsis

Lymphocytes from 48 patients with B-cell chronic lymphocytic leukemia (B-CLL) and allied disorders were examined with a panel of monoclonal antibodies and conventional surface marker techniques. Surface immunoglobulin (SIg) and Ia-like antigen were regularly present on B-CLL cells. In addition, 24 of 32 specimens reacted with OKTI, a monoclonal antibody which detects both peripheral and thymic T cells, but reactivity was not observed with anti-T-cell monoclonal antibodies of more restricted specificity (OKT3, OKT4, OKT6, OKT8 or OKTl 1). Eighteen of 20 samples in which only SIgM was detected were OKT 1 -positive, while all 4 with only SIgG were OKT 1 -negative. Cells from patients with hairy cell leukemia were unreactive with O K T l , but resembled B-CLL lymphocytes in the presence of clonal SIg and Ia-like antigen. Neoplastic plasma cells lacked Ia-like antigen and frequently SIg (2 of 9, but cytoplasmic immunoglobulin was present, cells reacted with OKTIO (replicating lymphoid cells) and 2 of 5 with OKT9 (transferrin receptor) as well. Cutaneous T-cell lymphoma samples were reactive with the anti-T-cell monoclonal antibodies OKT 1 OKT3 and OKT4 even when the sheep cell receptor could not be detected. Monoclonal antibodies can provide more certain diagnosis and superior resolution of cell lineage in these disorders than is possible by morphology alone.