Retinoblastoma gene deletions in b-cell chronic lymphocytic leukemia

✍ Scribed by Yie Liu; Dan Grandér; Stefan Einhorn; Stefan Söderhäll; Gunnar Juliusson; Gösta Gahrton

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 640 KB
• Volume: 4
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870040310

✦ Synopsis

Approximately 10% of B-cell chronic lymphocytic leukemia (B-CLL) cases have structural chromosomal aberrations involving band 13q14. To evaluate a possible role of RBI gene deletions in B-CLL we investigated the malignant cells of 27 patients by molecular genetic and cytogenetic techniques. Four of the cases had chromosomal aberrations that involved 13q I 4 (including one case with a 13q 14 deletion that was observed in a single metaphase cell), and I I had other chromosomal abnormalities, whereas the malignant cells of I 2 patients were either cytogenetically normal or failed t o divide in vitro. Eight patients (30%) were found to have hemizygous deletions of the RBI gene. These cases included all four patients with chromosomal changes at 13q14, but also three patients without chromosome abnormalities and one case with a chromosomal aberration not involving I3q. The deletions were interstitial in all cases but one, as defined by probes located centromeric and telomeric of the RBI locus, Inactivation of RBI may thus be a significant event in the development of some B-CLL clones. Genes Chrom Cancer 4:250-256 (I 992). @ 1992 Wiley-Liss. Inc.