Chromatographic recovery of polypeptides from copper-stained sodium dodecyl sulfate polyacrylamide gels
β Scribed by J.R. Vanfleteren; K. Peeters
- Book ID
- 118388053
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 705 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0165-022X
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π SIMILAR VOLUMES
We present a new method for visualizing proteins clectrophoresed in sodium dodecyl sulfate-polyacrylamide gels. After electrophorests. gels are incubated in CuCll to produce a negative image of colorless protein bands against a semiopaque background. Gels are stained completely within 5 min, do not
A simple method and apparatus are described for the efficient recovery of proteins from sodium dodecyl sulfate-polyacrylamide gel systems after electrophoretic resolution. This procedure provides for high yields of proteins which are free of sodium dodecyl sulfate and in certain cases, exhibit signi