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Copper staining: A five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels

✍ Scribed by Chris Lee; Andrew Levin; Daniel Branton

Book ID: 102988685
Publisher: Elsevier Science
Year: 1987
Tongue: English
Weight: 538 KB
Volume: 166
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(87)90579-3

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✦ Synopsis

We present a new method for visualizing proteins clectrophoresed in sodium dodecyl sulfate-polyacrylamide gels. After electrophorests. gels are incubated in CuCll to produce a negative image of colorless protein bands against a semiopaque background. Gels are stained completely within 5 min, do not require destaining, and can be stored indefinitely without loss ofthe image. Because proteins arc not permanently fixed within the gel. they can be quantitatively eluted after chelation of Cu with EDTA. The sensitivity of the CuCl, stain falls between that ofcoomassie blue and silver. We anticipate that CuCl, will be useful in the rapid analysis of proteins by polyacrylamide gel elcctrophoresis and in the preparation of purified polypeptidcs by elution from gel slices. c 1~x7 Acndcmlc I ' KSS.

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