Chlorophyta exclusively use the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate pathway for the biosynthesis of isoprenoids
✍ Scribed by Jörg Schwender; Claudia Gemünden; Hartmut K. Lichtenthaler
- Publisher
- Springer-Verlag
- Year
- 2001
- Tongue
- English
- Weight
- 150 KB
- Volume
- 212
- Category
- Article
- ISSN
- 0032-0935
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Free 2-C-methyl-d-erythritol is utilised for isoprenoid biosynthesis by Escherichia coli mutants lacking the two ®rst enzymes of the methylerythritol phosphate pathway, the deoxyxylulose phosphate synthase and isomero-reductase. For feeding experiments, this tetrol was synthesised with an overall 43
In the bacterium Escherichia coli, gcpE is an essential gene in the methylerythritol phosphate pathway for isoprenoid biosynthesis. Incubation of [1-3 H]methylerythritol with an E. coli mutant defective in the gcpE gene resulted in the accumulation of [1-3 H]methylerythritol 2,4-cyclodiphosphate. Th
Optically pure 2-C-methyl-d-erythritol 4-phosphate, a key intermediate in the mevalonate-independent route for isoprenoid biosynthesis, is conveniently synthesized from 1,2-O-isopropylidene-a-d-xylofuranose, including the possibility of radiolabeling of this substrate.