Homology modeling ofMycobacterium tuberculosis2C-methyl-d-erythritol-4-phosphate cytidylyltransferase, the third enzyme in the MEP pathway for isoprenoid biosynthesis
β Scribed by Cristian Obiol-Pardo; Alex Cordero; Jaime Rubio-Martinez; Santiago Imperial
- Publisher
- Springer-Verlag
- Year
- 2009
- Tongue
- English
- Weight
- 469 KB
- Volume
- 16
- Category
- Article
- ISSN
- 1610-2940
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π SIMILAR VOLUMES
Optically pure 2-C-methyl-d-erythritol 4-phosphate, a key intermediate in the mevalonate-independent route for isoprenoid biosynthesis, is conveniently synthesized from 1,2-O-isopropylidene-a-d-xylofuranose, including the possibility of radiolabeling of this substrate.
Optically pure 1-deoxy-D-xylulose, a key metabolite for feeding experiments in the methylerythritol phosphate pathway for isoprenoid biosynthesis, is conveniently synthesised from 1,2-O-isopropylidene-a-D-xylofuranose or from D-arabinose. This renders labelling with hydrogen isotopes possible.
In the bacterium Escherichia coli, gcpE is an essential gene in the methylerythritol phosphate pathway for isoprenoid biosynthesis. Incubation of [1-3 H]methylerythritol with an E. coli mutant defective in the gcpE gene resulted in the accumulation of [1-3 H]methylerythritol 2,4-cyclodiphosphate. Th