✦ LIBER ✦

Chemical and enzymatic synthesis of tritium labelled coenzymes

✍ Scribed by G. V. Sidorov; Yu. B. Zverkov; S. I. Shram; T. Yu. Lazurkina; N. F. Myasoedov

Publisher: John Wiley and Sons
Year: 2003
Tongue: French
Weight: 89 KB
Volume: 46
Category: Article
ISSN: 0022-2135
DOI: 10.1002/jlcr.688

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Details of the synthesis of tritium labelled co enzymes‐nicotinamide adenine dinucleotide and coenzyme A by isotope exchange and enzymatic reactions are reported. It was established, that among the investigated chemical reactions, the most effective is solid state isotope exchange with gaseous tritium. This method was used to produce [^3^H] nicotinamide adenine dinucleotide (111 Ci/mmol), [^3^H] coenzyme A (3.9 Ci/mmol) and D‐[G‐^3^H] pantothenic acid (43 Ci/mmol). It was shown that most of the tritium in the labeled nicotinamide adenine dinucleotide and coenzyme A was localized in the nicotinamide (98%) and adenine (89%) sites, respectively. For synthesis of coenzymes labelled with tritium at other sites we developed enzymatic methods which used labelled precursors. Optimum conditions for enzymatic synthesis of [adenine‐^3^H] nicotinamide adenine dinucleotide from [2,8‐^3^H] ATP and [pantothenate‐^3^H] coenzyme A from D‐[G‐^3^H] pantothenic acid were determined. The tritium labelled acetyl coenzyme A was synthesized by acetylation of labelled coenzyme A with acetic anhydride. The methods chosen allow one to produce tritium labelled coenzymes at high specific activity. Copyright © 2003 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Enzymatic synthesis of tritium-labelled

Enzymatic synthesis of tritium-labelled isotopomers of histamine

✍ E. Panufnik; R. Kański; M. Kańska 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 French ⚖ 92 KB

## Abstract Three tritium‐labelled isotopomers of histamine (HA) have been synthesized using combined chemical and enzymatic methods. In the first step the tritium‐labelled isotopomers of histidine have been obtained by catalysed exchange with tritiated water. These intermediates have been converte

Enzymatic synthesis of tritium-labelled

Enzymatic synthesis of tritium-labelled isotopomers of L-DOPA

✍ M. Kozłowska; R. Kański; M. Kańska 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 French ⚖ 107 KB

The synthesis of two isotopomers of l-DOPA labelled selectively with tritium is reported. In the intermediate step [3S-3 H]-, and [3 0 ,5 0 -3 H 2 ]-l-tyrosine, have been obtained using a combination of chemical and enzymatic methods. The labelled isotopomers of l-tyrosine, l-Tyr, have been converte

Enzymatic synthesis of N-methylhistamine

Enzymatic synthesis of N-methylhistamine labeled with deuterium and tritium

✍ J. Šamonina; M. Kańska 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 French ⚖ 154 KB

## Abstract The isotopomers of N^π^‐methylhistamine (πMeHA) and N^τ^‐methylhistamine (τMeHA) labeled with deuterium and tritium at the α‐carbon atom of the side chain were obtained using the enzyme histidine decarboxylase (HDC, EC 4.1.1.22) from __Lactobacillus 30a.__ The deuterium labeled isotopom

Enzymatic synthesis of isotopomers of ty

Enzymatic synthesis of isotopomers of tyramine labeled with deuterium and tritium

✍ Edyta Panufnik; Marianna Kañska 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 French ⚖ 116 KB

The combined chemical and enzymatic methods of synthesis of five isotopomers of l-tyrosine, l-Tyr, and their derivatives, i.e. corresponding isotopomers of tyramine (TA), labeled with deuterium and tritium have been reported. Two-step synthesis consists with introduction of deuterium or tritium labe

Enzymatic synthesis of phenylpyruvic aci

Enzymatic synthesis of phenylpyruvic acid labeled with deuterium, tritium, and carbon-14

✍ Katarzyna Skowera; Marianna Kańska 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 French ⚖ 115 KB

## Abstract The synthesis of isotopomers of phenylpyruvic acid, PPA, selectively labeled with hydrogen isotopes in the 3‐position of the side‐chain is reported. Three deuterium or tritium labeled isotopomers of L‐phenylalanine, L‐Phe, i.e. [(3__S__)‐^2^H]‐L‐, [(3__S__)‐^3^H]‐L‐, and doubly labeled

Synthesis of tritium labelled precocenes

✍ A. Banerji; N. C. Goomer 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 French ⚖ 200 KB 👁 1 views

## Abstract Regiospecific syntheses of tritium labelled precocene I (**5a**) and precocene II (**5b**), the antijuvenile hormones, have been accomplished. The syntheses involved condensations between 2‐hydroxyacetophenones (**1**) with acetone‐T to give cross condensation products **2** which on cy