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Biosynthesis of [7-3H]16α-hydroxy-dehydroepiandrosterone having high specific activity

✍ Scribed by Dr. M. Iida; Dr. K. Matsuhashi; T. Nakayama

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 280 KB
Volume: 15
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.19750150307

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✦ Synopsis

Abstract

Biosynthesis of [7‐^3^H]16α‐hydroxy‐dehydroepiandrosterone in high specific activity has been studied. [7‐^3^H] dehydroepiandrosterone (13.9 C/mM) in trace quantity was oxidized by Streptomyces roseochromogenes (NRRLB‐1233) for 5 min at 27 °C. The radioactive products were chromatographically separated, identified and their radiochemical purity established by isotopic dilution analysis. [7‐^3^H]16α‐hydroxy‐dehydroepiandrosterone (2.5 × 10^7^ dpm) was obtained by microbial hydroxylation of substrate (1.9 × 10^9^ dpm). In some cases [7‐^3^H]5‐androstene‐3β, 16α, 17β‐triol in a small amount of radioactivity could be found at the prolonged reaction for 30 hr.

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