with the identity test based upon the evolution of iodine vapor and comparison of the infrared spcctrurn provide a satisfactory identification of idoxuridine. Purity Tests.-Thin-layer or paper chromatographic procedures may be included for testing the purity of bulk idoxuridine. Comparison of idoxu
AUTOMATION OF THE LIVER GLYCOGEN DETERMINATION
β Scribed by Frank M. Singer; Aleck Borman; Leonard J. Lerner
- Book ID
- 114876831
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 713 KB
- Volume
- 102
- Category
- Article
- ISSN
- 0890-6564
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A procedure developed in this laboratory for the enzymic determination of glycogen was described earlier (1, 2). Krebs and co-workers (3) reported a similar procedure at about the same time, and have been employing it for the analysis of carbohydrate in tissue extracts (4). The t,echnique employs a
A method is proposed to measure glycogen content in liver homogenates without extraction and acid hydrolysis of tissue glycogen. Homogenates were treated with amyloglucosidase, which degrades glycogen to glucose, and the glucose was then determined enzymatically by the use of glucose oxidase and per