A method is proposed to measure glycogen content in liver homogenates without extraction and acid hydrolysis of tissue glycogen. Homogenates were treated with amyloglucosidase, which degrades glycogen to glucose, and the glucose was then determined enzymatically by the use of glucose oxidase and per
A Simple Method for the Determination of Glycogen in Liver
β Scribed by GYERMEK, L.; FEKETE, G.
- Book ID
- 109581166
- Publisher
- Nature Publishing Group
- Year
- 1955
- Tongue
- English
- Weight
- 118 KB
- Volume
- 175
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/175386a0
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A method for enzymatic glycogen determination in yeast cells without need for prior disruption of the cell walls has been developed: After stopping cell metabolism with 5% trichloroacetic acid, the cells were digested with 0.25 M Na&O, for 90 min on a boiling water bath and pH was adjusted to 4.8. G
A procedure developed in this laboratory for the enzymic determination of glycogen was described earlier (1, 2). Krebs and co-workers (3) reported a similar procedure at about the same time, and have been employing it for the analysis of carbohydrate in tissue extracts (4). The t,echnique employs a