Direct enzymatic procedure for the determination of liver glycogen

✍ Scribed by Karia L. Roehrig; John B. Allred

• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 458 KB
• Volume: 58
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(74)90210-3

✦ Synopsis

A method is proposed to measure glycogen content in liver homogenates without extraction and acid hydrolysis of tissue glycogen. Homogenates were treated with amyloglucosidase, which degrades glycogen to glucose, and the glucose was then determined enzymatically by the use of glucose oxidase and peroxidase. The method was shown to yield nearly complete (99%) recoveries of standard glycogen, while 5 hr of acid hydrolysis of standard glpcogen were required to obtain comparable recoveries. When compared to an acid hydrolysis met,hod for liver, amyloglucosidase degradation of rat liver glycogen and subsequent determination of glucose resulted in higher values for glycogen content. The amyloglucosidase, glucose oxidase: peroxidase method has the advantage of rapidity, whereas the traditional method consisting of extraction, precipitation, and acid hydrolysis is not only time consuming, but may also be subject to losses of glycogen in each step. 414