Photometric determination of glycogen in stained liver sections
✍ Scribed by Björn Sandström
- Book ID
- 112289393
- Publisher
- Springer
- Year
- 1971
- Tongue
- English
- Weight
- 701 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1432-119X
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📜 SIMILAR VOLUMES
A procedure developed in this laboratory for the enzymic determination of glycogen was described earlier (1, 2). Krebs and co-workers (3) reported a similar procedure at about the same time, and have been employing it for the analysis of carbohydrate in tissue extracts (4). The t,echnique employs a
A method is proposed to measure glycogen content in liver homogenates without extraction and acid hydrolysis of tissue glycogen. Homogenates were treated with amyloglucosidase, which degrades glycogen to glucose, and the glucose was then determined enzymatically by the use of glucose oxidase and per