Automated nephelometric determination of rat liver glycogen in adrenal steroid bioassays

✍ Scribed by William F. Beyer

• Publisher: John Wiley and Sons
• Year: 1966
• Tongue: English
• Weight: 314 KB
• Volume: 55
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600550620

✦ Synopsis

with the identity test based upon the evolution of iodine vapor and comparison of the infrared spcctrurn provide a satisfactory identification of idoxuridine.

Purity Tests.-Thin-layer or paper chromatographic procedures may be included for testing the purity of bulk idoxuridine. Comparison of idoxuridine to a reference standard is made by examination of dcvelopcd chromatograms using an ultraviolet light and/or color producing reagents, i.e., cysteine-sulfuric acid. The idoxuridinc spot should be equivalent in position to the reference standard spot for idoxuridinc, and no other spots a t othcr positions should be visible. The spotting of control solutions containing the degradation products of idoxuridine (5-iodouracil, uracil, and deoxyuridine) will aid in detecting the position of extraneous spots on the chromatograms.

Quantitative Methods.-The quantitative determination of the iodine content of idoxuridine is similar to the official assay for sodium liothyronine

( 3 ) and gave an average valuc equivalent to 36.1 f O.lYA3 iodine. A rapid, precise measure of the iodine content may also be determined by the oxygen flask method (4-6). Nonaqueous titration of idoxuridine with sodium methoxidc gavc an average value of 99.8 + 0.6'%.3 &o violet indicator may also be used for the end point detection of the titration. Analysis of the sterile ophthalmic solutions by column partition chromatography was