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Automated Detection of Anti-Double-Stranded DNA Antibody in Systemic Lupus Erythematosus Serum by Flow Immunoassay

โœ Scribed by Lim, Tae-kyu; Komoda, Yumiko; Nakamura, Noriyuki; Matsunaga, Tadashi

Book ID
125931750
Publisher
American Chemical Society
Year
1999
Tongue
English
Weight
98 KB
Volume
71
Category
Article
ISSN
0003-2700

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An anti-double-stranded (ds) DNA antibodies in serum-detection system was developed based on flow immunoassay with packed-capillary column. Alkaline phosphatase conjugated DNA (ALP-DNA) and anti-dsDNA antibody were separated from unreacted ALP-DNA on the basis of the difference in isoelectric point.