Detection of low avidity anti-dna antibodies in systemic lupus erythematosus
β Scribed by Richard L. Riley; Hugh Mcgrath Jr.; Ronald P. Taylor
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 616 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0004-3591
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## Abstract Twentyβone sera from 11 patients with systemic lupus erythematosus were titrated with ^125^Iβssβcalf thymus DNA to determine their maximum capacity to bind DNA. Only five sera were capable of binding > 90% of the input DNA. The remaining sixteen sera showed maximum DNA binding levels be
Antibody a c t i v i t y t o various histones was examined i n a s o l i d phase radioimmunoassay. More than one h a l f o f the sera from systemic lupus erythematosus (SLE) p a t i e n t s had IgM a c t i v i t y t o t o t a l histones, and a s l i g h t l y lower frequency was observed f o r IgG a
Sera from patients with SLE frequently contain IgM and IgG antibodies with multiple specificities for lymphocyte surface determinants, including autologous antigens. The IgM antibodies are of relatively low binding avidity and exhibit broad reactivity with B and T lymphocytes from most individuals.
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