Use of anion exchange resin-packed capillary column for rapid detection of anti-double-stranded DNA antibody in systemic lupus erythematosus serum
✍ Scribed by Tae-kyu Lim; Noriyuki Nakamura; Tadashi Matsunaga
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 118 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
An anti-double-stranded (ds) DNA antibodies in serum-detection system was developed based on flow immunoassay with packed-capillary column. Alkaline phosphatase conjugated DNA (ALP-DNA) and anti-dsDNA antibody were separated from unreacted ALP-DNA on the basis of the difference in isoelectric point. A linear anti-dsDNA antibody dose-response curve was obtained between luminescence intensity and concentration of anti-dsDNA antibody in the range 25-200 IU/mL. This simple technique permits the assay of anti-dsDNA autoimmune antibodies within 7 minutes. These results give reduced detection times compared with those previously reported (Lim et al., 1999) through the use of capillary columns.