## Abstract Abortive transformation of layer cultures of BHK 21 cells by polyoma virus has been studied using serumโdepleted medium. High doses of partially purified infectious virus, in the absence of serum, stimulate the majority of the cells to synthesize DNA while empty capsids do not. The onse
AScites Tumours From BHK.21 Cells Transformed in Vitro by Polyoma Virus
โ Scribed by SANDERS, F. K.; BURFORD, BARBARA O.
- Book ID
- 109634313
- Publisher
- Nature Publishing Group
- Year
- 1964
- Tongue
- English
- Weight
- 618 KB
- Volume
- 201
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/201786a0
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## Abstract When BHK 21 cells are exposed to high multiplicities of polyoma virus, a change in surface antigen may be detected by immunofluorescence, beginning at 18 h and affecting up to 88% of cells 24 h after infection. Timeโlapse cinematography shows that there is a concurrent change in movemen
Some features of the metabolism of untransformed and polyoma-transformed clones of the BHKZI cell strain were studied. I ) No difference in the oxygen uptake of the two groups was detected but in all cases the transformed clones had greater glycolytic capaci!y than the untransformed clones at p H 7.
## Abstract The transformation of cultivated hamster brain cells by polyoma virus is reported. The transformed cell line contained polyoma virusโspecific nuclear, surface and transplantation antigens. Subcutaneous and intracranial inoculations revealed high tumorigenicity of the cells. Brainโspecif