Increased glycolytic capacity and associated enzyme changes in BHK21 cells transformed with polyoma virus
✍ Scribed by J. Paul; M. M. Broadfoot; P. Walker
- Publisher
- John Wiley and Sons
- Year
- 1966
- Tongue
- French
- Weight
- 619 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Some features of the metabolism of untransformed and polyoma-transformed clones of the BHKZI cell strain were studied. I ) No difference in the oxygen uptake of the two groups was detected but in all cases the transformed clones had greater glycolytic capaci!y than the untransformed clones at p H 7.4, in both aerobic and unaerobic conditions. 2 ) The transformed clones had more hexokinase and isocitric dehydrogenase, and probably more malic dehydrogenase, than the untransformed clones. 3 ) The untransformed clones had more glucose-6-phosphate dehydrogenase than the transformed clones. 4 ) No differences could be detected in 6-phosphogluconate dehydrogenase or lactic dehydrogenase activity. 5 ) It is suggested that these differences may be related either to diflerences in the permeability of the cell membranes to glucose or to an altered rate of synthesis of mucopo1.vsaccharides. * Activity measured by the method o f DiPietro and Weinhouse (1960).